Relevance
In contemporary clinical practice, there i no optimal empirical therapy for H. pylori infection and there is a progressive decrease in the effectiveness of classical eradication therapy regimens. The new version of the MAASTRICHT VI consensus of 08.08.2022 introduced a provision on molecular diagnostic methods (in particular, real-time PCR, genome-wide sequencing and digital PCR) to detect H. pylori mutations associated with resistance to clarithromycin, levofloxacin, tetracycline and rifampicin considering the increasing antibiotic resistance. Furthermore, before administering any therapy regimens containing clarithromycin, it is recommended to conduct a clarithromycin sensitivity test using molecular or bacteriological methods, if available. For molecular testing using PCR, it is proposed to use stomach biopsies extracted from rapid urease tests (rapid urease test). Genotypic and phenotypic markers of H. pylori are recommended to be used for personalized eradication therapy.
Detection of H. pylori, determination of sensitivity to antimicrobial drugs by classical microbiological methods is a complex process that requires certain competencies and it is not always a guarantor of the detection of the pathogen. As a result, more and more laboratories are focusing on molecular genetic methods in the diagnosis of H. pylori. After all, they allow you to analyze the structure of the pathogen, its genome, mutations, virulence genes, antimicrobial resistance genes.
In addition, research concerning the search for phylogenetic lines is becoming increasingly popular. The resulting H. pylori may have phylogeographic differentiation. The genotypes of the studied H. pylori can potentially be used as markers of migration of human populations.
Asymptomatic carriers of H. pylori may be at risk for the development of chronic processes in the gastrointestinal tract, up to cancerous conditions. Knowing the genetic variability of the pathogen, it is possible to predict the course of the disease.
Expected results
Based on the results of this study, it is planned to identify the circulation of resistant strains and the dynamics of their spread, to identify groups with certain genotypes resistant to standard schemes of anti-helicobacter therapy. It is planned to obtain the first data in Kazakhstan on the population genetics of H. pylori.
For the first time we will try to determine the regional features of genotypes H. pylori in Kazakhstan. Data will be obtained on the effectiveness or inefficiency of the classical cultural and molecular genetic method in the diagnosis of H. pylori. Data on the identification of H. pylori on MALDI-TOF with the creation of its own local libraries will be obtained.
Data on molecular genetic predictors of H. pylori resistance to eradication therapy regimens in the Republic of Kazakhstan will be obtained.
The concept of the fundamentals of population genetics of H. pylori will allow for a deeper study of the origin of the strain, providing a new way to understand the information and mechanisms of antibiotic resistance. All this will help to develop up-to-date diagnostic approaches for predicting pathogenic and non-pathogenic strains in the development of gastric ulcer, duodenal ulcer or adenocarcinoma. This project has a potential socio-economic effect, which consists in choosing the optimal method for diagnosing H. pylori, determining sensitivity to antimicrobial drugs in Kazakhstan, as well as determining regional genotypes and levels of resistance to antibacterial drugs when planning therapeutic strategies for H. pylori infection.
H.pylori, определения чувствительности к антимикробным препаратам на территории Казахстана, а также, определения региональных генотипов и уровней резистентности к антибактериальным препаратам при планировании терапевтических стратегий инфекции H.рylori.
The obtained data on the population genetics of H. pylori in Kazakhstan will make it possible to make changes to the diagnostic algorithm with the further prospect of finalizing the treatment concept according to the global trends of personalized medicine. The relevance of the obtained results will be applied both for applied clinical tasks and in the field of fundamental medicine. The areas of application of the research results and target consumers include: pharmacology, oncology, immunology, clinical microbiology, laboratory diagnostics, therapy, gastroenterology.
Based on these results, it is planned to publish at least 2 (two) at least 2 (two) articles and (or) reviews in peer-reviewed scientific publications in the scientific direction of the project, indexed in the Science Citation Index Expanded of the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 35 (thirty-five), at least 1 (one) article or review in a peer-reviewed foreign or domestic publication, recommended by CQAFSHE.
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Research Group
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№ |
Full name, Education, degree academic title |
Main place of work, position |
The hirsch index, ResearcherID identifiers, ORCHID, Scopus Author ID |
Role in the project or program and nature of the work performed |
List of publications |
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1 |
Ekaterina Alexandrovna Yukhnevich, PhD, Associate Professor |
NАО "KMU", associate professor |
The hirsch index– 2 Web of Science Researcher ID AAT-1398-2020 ORCID ID 0000-0001-5992-0336– 2 Web of Science Researcher ID AAT-1398-2020 ORCID ID 0000-0001-5992-0336 |
Scientific director Project manager, coordinator. General project management, coordination of group activities, task monitoring, data analysis, publication writing.
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1. G. Kemelova; I. Shekhter; D.Aimbetova; V.Riklefs; Y. Yukhnevich. Digitalization of the clinical exam in Covid-19 pandemic: Karaganda Medical University’s experience//Journal of Clinical Medicine of Kazakhstan 2022-04-30 | Journal article DOI: 10.23950/jcmk/11942 2. D.Yesniyazov; Tussupbekova M.; N.Abatov; Y. Yukhnevich; R.Badyrov Myringoplasty with Morphological Rationale of Application of Xenoperitoneum Decellularized Matrix in Experiment// Open Access Macedonian Journal of Medical Sciences 2021-10-05 | Journal article 3. D.Kaliyeva; Y.Yukhnevich; N.Abatov; A. Nurbekov., Difficulties in diagnosing the alveolar echinococcosis (case report)., International Journal of Surgery Case Reports 2020 | Journal article DOI: 10.1016/j.ijscr.2020.08.025 S Kaliyeva; N Simohina; Y Yukhnevich; Z Myasnikova; Y Myasnikov; Yekaterina Yukhnevich., Pharmacoeconomic Assessment of Biological Therapy of Ulcerative Colitis, Value in Health 2018-05 | Conference abstract | Writing - original draft, Methodology DOI: 10.1016/j.jval.2018.04.567 Begaidarova,R.; Kaliyeva, S.; Yukhnevich, Y.; Tonkabaeva, B.; Begey, N., SAUSALIN VS METRONIDAZOLE IN TREATMENT OF GIARDIASIS: PHARMACOECONOMIC ASSESSMENT, Value in Health 2017 | Journal article WOSUID: WOS:000405448001075 Begaydarova,R.; Yukhnevich, Y.; Babenko, D.; Kaliyeva, S.; Azizov, I.; Muldaeva, G.; Omarkulov, B., Determination of PFOR gene expression in strains of G. intestinalis with different inhibitory concentrations of metronidazole., Journal of Infection in Developing Countries 2015 | Journal article DOI: 10.3855/jidc.5768 Begaydarova,R.H.; Kultanov, B. Z.; Esilbaeva, B.T.; Nasakaeva,G.E.; Yukhnevich,Y.A.; Alshynbekova, G. K.; Dyusembaeva, A. E., The investigation of endogenous intoxication and lipid peroxidation in patients with giardiasis before and after treatment, The FEBS Journal 2015 | Journal article WOSUID: WOS:000362570602099 S Kalyieva; N Minakova; Y Yukhnevich-Nassonova., Cost-Effectiveness Analysis of the Original Drug Arglabin., Value in Health 2014-11 | Journal article DOI: 10.1016/j.jval.2014.08.103 Begaydorova RKh; Nasakaeva GE; Tabagari SI; Alshinbekova GK; Yekaterina Yukhnevich., Clinical and diagnostic features and treatment of giardiasis, Georgian medical news 2014-11-01 | Journal article PMID: 25541827 Yekaterina Yukhnevich; Begaydarova, R; Kultanov,B.Zh; Yukhnevich-Nassonova, Y.; Kuzgibekova, A; Nasakaeva, G,. Evaluation of cytogenetic changes in somatic cells in patients with giardiasis (Definition of micronucleus test in somatic cells - cells with buccal mucosa), Global Journal on Advances in Pure & Applied Sciences 2014-04 | Journal article |
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2 |
Lavrinenko Alyona Vladimirovna |
NАО KMU, Associate Professor, Head of the laboratory |
The hirsch index– 3 R ID - 20,56 ORCID 0000-0001-9436-8778. Scopus Author ID 57193763117 |
Responsible executor. Coordinator, collection of material, microbiological part of the work, writing publications |
1. Bacterial Co-Infections and Antimicrobial Resistance in Patients Hospitalized with Suspected or Confirmed COVID-19 Pneumonia in Kazakhstan. Lavrinenko, A., Kolesnichenko, S., Kadyrova, I., Akhmaltdinova, L. Klyuyev, D. Pathogens, 2023, 12(3), 370. 2. The Presence of PDL-1 on CD8+ Lymphocytes Is Linked to Survival in Neonatal Sepsis. Akhmaltdinova, L.L., Zhumadilova, Z.A., Kolesnichenko, S.I., Panibratec, L.G., Vinogradskaya, E.V.. Children, 2022, 9(8), 1171. 3. Bacterial Colonization Incidence before and after Indwelling Double-J Ureteral Stents. Kaliyeva, S., Simokhina, N., Lavrinenko, A., Yelmagambetov, B., Myasnikova, Z.. Antibiotics, 2022, 11(7), 850. 4. ANTIBIOTIC RESISTANCE AND GENOTYPES OF NOSOCOMIAL STRAINS OF ACINETOBACTER BAUMANNII IN KAZAKHSTAN. Lavrinenko A., Kolesnichenko S., Turmukhambetova A., Sheck E., Azizov I. Antibiotics. 2021. Т. 10. № 4. 5. Mortality Risk Factors of Early Neonatal Sepsis During COVID-19 Pandemic. Kolesnichenko, S.I., Kadyrova, I.A., Lavrinenko, A.V., Panibratec, L.G., Akhmaltdinova, L.L. Infection and Drug Resistancethis link is disabled, 2022, 15, страницы 6307–6316 Antibiotics, 2021, 10(4), 382. 6.BLOODSTREAM INFECTION ETIOLOGY AMONG CHILDREN AND ADULTS. Kolesnichenko S.I.,. Lavrinenko A.V., Akhmaltdinova L.L. International Journal of Microbiology. 2021. Т. 2021. С. 6657134. 7. INFLUENCE OF PATHOGEN TYPE ON NEONATAL SEPSIS BIOMARKERS. Akhmaltdinova L., Kolesnichenko S., Lavrinenko A., Kadyrova I., Avdienko O., Panibratec L. International Journal of Inflammation. 2021. Т. 2021. С. 1009231. 8.STUDY OF THE PHENOLIC COMPOUNDS OF THE DRY EXTRACT OF THYMUS CREBRIFOLIUS USING A COMBINED HPLC-UV AND HPLC-ESI-MS/MS METHOD. Ivasenko S.A., Shakarimova K.K., Bokayeva A.B., Marchenko A.B., Lavrinenko A.V., Kolesnichenko S.I. Bulletin of the Karaganda University. Chemistry Series. 2021. № 2 (102). С. 18-23. 9.СОСТОЯНИЕ АНТИБИОТИКОРЕЗИСТЕНТНОСТИ ВОЗБУДИТЕЛЕЙ ВНЕБОЛЬНИЧНЫХ ИНФЕКЦИЙ МОЧЕВЫВОДЯЩИХ ПУТЕЙ В РОССИИ, БЕЛАРУСИ И КАЗАХСТАНЕ: РЕЗУЛЬТАТЫ МНОГОЦЕНТРОВОГО МЕЖДУНАРОДНОГО ИССЛЕДОВАНИЯ «ДАРМИС-2018» Палагин И.С., Сухорукова М.В., Дехнич А.В., Эйдельштейн М.В., Перепанова Т.С., Козлов Р.С., Мутин М.Ю., Стребкова В.В., Тапальский Д.В., Аминева П.Г., Ветохина А.В., Сухорева М.В., Иванова И.А., Валиуллина И.Р., Лавриненко А.В., Частоедова А.Н., Широкова Т.М., Варибрус Е.В., Васильева И.Р., Доманская О.В. и др. Урология. 2020. № 1. С. 19-31. 10.ПНЕВМОНИИ, АССОЦИИРОВАННЫЕ С SARS-COV-2. Лавриненко А.В., Колесниченко С.И., Турмухамбетова А.А., Кадырова И.А. Вестник Казахского национального медицинского университета. 2020. № 4. С. 301-307. 11.СПЕКТР И ЛЕКАРСТВЕННАЯ ЧУВСТВИТЕЛЬНОСТЬ НЕСПЕЦИФИЧЕСКОЙ ФЛОРЫ У БОЛЬНЫХ ТУБЕРКУЛЕЗОМ С МНОЖЕСТВЕННОЙ ЛЕКАРСТВЕННОЙ УСТОЙЧИВОСТЬЮ Лавриненко А.В., Азизов И.С., Колесниченко С.И., Кожамуратов М.Т., Табриз Н.С., Мутайхан Ж.
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3 |
Kadyrova Irina Adilyevna, PhD |
NАО "KMU", senior resercher |
The hirsch index – 4 ORCID 0000-0001-7173-3138 Scopus 56297966100 Publons V-8868-2019 |
Performer molecular genetic research, statistical analysis, analysis of data obtained, writing publications
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1. Biomarkers of immunothrombosis and polymorphisms of IL2, IL6, and IL10 genes as predictors of the severity of COVID-19 in a Kazakh population/ Yessenbayeva, A., Apsalikov, B. Massabayeva, M., Aukenov, N..// PLoS ONEthis link is disabled, 2023, 18(6 JUNE), e0288139. 2. Bacterial Co-Infections and Antimicrobial Resistance in Patients Hospitalized with Suspected or Confirmed COVID-19 Pneumonia in Kazakhstan. Lavrinenko, A., Kolesnichenko, S. Kadyrova, I., Akhmaltdinova, L., Klyuyev, D. Pathogens, 2023, 12(3), 370 3.Genetic Predictors of the Development of Complications after Coronary Stenting.Taizhanova, D., Kalimbetova, A., Bodaubay, R., Akilzhanova, A., Gerotziafas, G.T. Journal of Personalized Medicine, 2023, 13(1), 14. 4. Sputnik-V reactogenicity and immunogenicity in the blood and mucosa: a prospective cohort study
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4 |
Solomadin Maxim Valeryevich, Master |
NАО "KMU", 3rd year doctoral student |
ORCID ID 0000-0002-1400-3267 |
Performer Molecular genetic research, analysis of laboratory data.
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G.Kairova; N.Daulet; M. Solomadin; N. Sandybayev; S. Orkara; V. Beloussov; N.Kerimbek; D.Gritsenko; Z.Sapakhova,.Identification of Apple Varieties Resistant to Fire Blight (Erwinia amylovora) Using Molecular Markers., Horticulturae 2023-09-04 | Journal article DOI: 10.3390/horticulturae9091000 B.S. Ilyassova; B.Abzhaparova; D. S. Smailova; A.Bolatov; B.Baymakhanov; V.Beloussov; M.Solomadin; K. Shamsivaliyeva; G.Alpysbayava; G. Issakova et al., Prevalence and genotypes distribution of virus hepatitis B and hepatitis delta virus in chronic liver diseases in Kazakhstan., BMC Infectious Diseases 2023-08-14 | Journal article DOI: 10.1186/s12879-023-08524-1 N.Sandybayev; V.Beloussov; V. Strochkov; M.Solomadin; J. Granica; S. Yegorov., Characterization of viral pathogens associated with symptomatic upper respiratory tract infection in adults during a low COVID-19 transmission period., PeerJ 2023-03-13 | Journal article DOI: 10.7717/peerj.15008 N.Sandybayev; V. Beloussov; Vitaliy Strochkov; M.Solomadin; J.Granica; S.Yegorov., Next Generation Sequencing Approaches to Characterize the Respiratory Tract Virome., Microorganisms 2022-11-24 | Journal article DOI: 10.3390/microorganisms10122327 Lavrinenko, A. V.; Azizov, I.; Solomadin, M.; Kolesnichenko, S.., Molecular-genetic features of MSSA, isolated in Kazakhstan., International Journal of Infectious Diseases 2018 | Journal article DOI: 10.1016/J.IJID.2018.04.3773 Kalimagambetov, A.; Isabek, A.; Valyayeva, M.; Rakisheva, Z.; Beisembaeva, S.; Sadueva, K.; Solomadin, M., Polymorphism of thrombophilia genes of the blood coagulation system in women of the Kazakh ethnic group with a habitual miscarriage., The FEBS Journal 2017 | Journal article WOSUID: WOS:000409918902086 The FEBS Journal 2017 | Journal article WOSUID: WOS:000409918902086., THE TRANSMISSIVE ROUTE OF BACILLUS ANTHRACIS INFECTION., The American Journal of Tropical Medicine and Hygiene 2010 | Journal article WOSUID: WOS:000295819700194 |
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5 |
Alexey Pavlovich Shkreba |
NАО "KMU", endoscopist |
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Performer Performing endoscopy, collecting material
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6 |
Simokhina Natalia Andreevna |
NАО "KMU", assistant professor |
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Performer Clinical part of the work, monitoring the effectiveness of eradication regimens, statistical analysis, writing publications
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7 |
Ryazanets Darya Anatolyevna |
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8 |
Turemuratova Aidana Bauyrzhanovna |
NАО "KMU", trainee research |
ORCID 0000-0003-2322-304X |
Исполнитель Участие в постановке культурального метода, определение чувствительности к антибиотикам
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1.Лавриненко А.В., Туремуратова А.Б., Пралиева А.Б. Определение чувствительности к колистину методом микроразведений в бульоне с использованием стандартных дисков -// журнал Клиническая Микробиология и Антимикробная Химиотерапия. – Том 24, 2022 . С 23. ISSN 1684-4386
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The process of cultivation, identification and preservation of samples has begun. Also, a stage of work has begun to determine the sensitivity of H. pylori to antimicrobial drugs.
The results of the study will be useful in the diagnosis of H. pylori in gastroenterology.
